Design of Species-Specific PCR Primers That Target the aac(6′)-Ii Gene for the Rapid Detection of Enterococcus faecium

In this study, we used the sequence of aac(6′)-Ii gene, which is responsible for intrinsic low-level aminoglycoside resistance Enterococcus faecium, to design novel species-specific primers. Two oligonucleotide pairs named EF_200 and EF_120 were designed, generating amplification products with sizes 200 bp 120 bp, respectively. They successfully applied identification various isolates clinical or environmental origins in both pure cultures complex food samples. The obtained results indicated that primer permitted highly specific, simple, fast inexpensive detection E. faecium isolates.